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Molecular Genetic Fingerprint of some Streptomyces Isolated
from Riyadh city
QAl-Kahtani Munira &AI-Khalil Abdullah &
AI-Kadeeb Siham &Hassan H.Z.
Department of Botany, Girls College of Education,
Riyadh, P.O .Box 27104, Riyadh 11417, Saudi Arabia
Abstract
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The present investigation was conducted to develop molecular
fingerprints for fifteen Streptomyces isolates gathered from Riyadh city
by using RAPD-PCR. Three 10-mer random primers (OPB-16, OPF-05 and
OPK-10) out of eight had successfully generated reproducible polymorphic
products. Each of the three primers displayed a strong amplification
with distinct bands. The fingerprints generated by these primers
revealed characteristic profiles for each of the fifteen Streptomyces
isolate, in terms of number and position of RAPD bands. Primer OPB-16
generates a total of 19 polymorphic bands. Two of these polymorphic
bands were identified as unique ones. While the remaining ones were
detected as 17 non unique bands. The generated RAPD profile with the
primer OPF-05 shows a total of 23 polymorphic bands among the studied
isolates. It comprises one unique band and 22 non unique polymorphic
ones. Similarly, primer OPK-10 develops 14 polymorphic bands which
scored as 4 unique and 10 non unique bands. The unique bands were used
to discriminate between some of the studied Streptomyces isolates. In
addition, the other recorded polymorphic bands (non unique) provided a
considerable number of combined class patterns that completly
discrimination between all the studied isolates.
The results generated from RAPD profiles were pooled for drawing the
genetic relationships among the examined Streptomyces species. The
constructed dendrogram tree divided the fifteen isolates into two
groups. The highest similarity value (0.851) was observed between the
two isolates no. 13 and 14. On the other hand, the lowest similarity
value (0.000) was scored between the two isolates no. 5 and 6.
Key words: Streptomyces, RAPD-PCR, Fingerprint, Polymorphism,
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