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Improved DNA Extraction Method for Porcine Contaminants,
Detection in Imported Meat to The Saudi Market
Ibrahim Abdullah Alaraidh
Biology Department, Teachers College, King Saud University. P. O. 4341,
Riyadh 11491, Saudi Arabia.
E-mail: ibrahim1551@hotmail.com
Abstract _______________________________________________________________________________________________________
A porcine detection methodology based on deoxyribonucleic acid (DNA)
extraction and polymerase chain reaction (PCR) amplification of a
specific porcine fragment was used in this paper. With the advent of
mass globalization and the fast growing and rapidly changing halal
industry of the international market it is of vital need that a
practical scientific system be applied and established in the Kingdom of
Saudi Arabia to detect and monitor the ingredients in food especially
porcine contaminants. A simple modified technique was designed to
extract DNA from food material. ASL buffer (Qiagen) for lysis and a
series of incubation steps were applied prior to PCR and detection
limits were established. The optimized method was then used to identify
pork in food products obtained from different local hypermarkets. The
used method was displayed to be robust and reliable. Out of thirty-three
food samples labeled as halal, there were two imported samples; one beef
steak and one beef sausage that were positive for porcine contaminants.
From the results it can be concluded that sufficient PCR ready DNA can
be obtained by this single solution methodology. Although the incubation
for the lyses is recommended to be overnight the cost of the experiment
and the relative simplicity and reliability of the experiment allows a
mass food testing ability and a viable commercialization opportunity in
the Kingdom. It is also of absolute importance that a systematic
scientific precaution be taken to ensure that imported food products in
Saudi Arabia are actually halal, as Allah says in the Holy Qur’an:
“Forbidden to you (for food) are: Al-Maitah (the dead animals - cattle -
beast not slaughtered), blood, the flesh of swine” (Sűrat Al-Mâ’idah,
Verse 3).
Key Words: Porcine, DNA, PCR, Primer, Processed meat, Detection,
halal.
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