|
Saudi Journal of Biological Sciences 15 (2) 189-198
December, 2008 |
||
|
Abstract In vitro, susceptibilities
were determined for 107 clinical isolates of Candida albicans obtained
from the laboratory of King Abdulaziz University Hospital (UHC). The
agents tested included amphotericin B (as a polyene), flucytosine (as a
pyrimidine), and fluconazole (as an azole). MICs were determined by the
broth microdilution technique following National Committee for Clinical
Laboratory Standards document, using RPMI 1640 broth medium supplemented
with 3 g glucose l-1, and the E-test with solidified RPMI 1640
supplemented with 18 g glucose l-1. The susceptibility tests indicated
that amphotericin B is the most active fungicidal drug, fluconazole was
with less activity and flucytosine was more effective than the later
drug. The appropriate rank order of best agreement between the MIC
technique, E-test and disk diffusion methods was amphotericin B>flucytosine>
fluconazole. The influence of the tested drugs in adherence of
C.albicans on phenyl sepharose (resin) column indicated that
amphotericin B was with high antiadherence activity, followed by
fluconazole and flucytosine (47, 33 and 18%, respectively.) The effect
of the drugs on mycelial formation by the tested C.albicans isolates
revealed that amphotericin B showed 71% inhibition of mycelial
formation, fluconazole 58% and flucytosine showed about 40% inhibition
of mycelial formation. ______________________________________________________________________________________________________ |
||