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Histochemical Characterization of the Cytoplasmic
Inclusions Induced by Chronic Lead Toxicity
Bashir M. Jarrar and Maher M. Al-Enazi
Department of Clinical Laboratory Sciences,College of Applied Medical
Sciences, Al-Jouf University, Saudi Arabia
Abstract _______________________________________________________________________________________________________
This study was designed to characterize the nature of the cytoplasmic
inclusions induced by chronic lead toxicity. A battery of histological
and histochemical techniques was applied on these inclusions induced in
the kidneys of adult male rabbits received 1% lead acetate in their
drinking water. The results proved that lead cytoplasmic inclusions are
heterogeneous deposits contain lead, basic proteins, neutral and acidic
lipids, and unsaturated fats but devoid of nucleic acids. The staining
ability of these inclusions proved that they are basic in nature
containing positive roots in their auxochromes, and having reduction
ability. These inclusions have shown affinity towards anionic dyes that
carry acidic radicals with no affinity towards cationic dyes. These
inclusions have shown orthochromatic, argyrophilic, osmophilic,
fuchsinophilic and sudanophilic affinities but no alcianophilia. The
results are discussed in the context of lead cytoplasmic inclusions
protection function from lead toxicity.
Key Words : Lead ; cytoplasmic inclusions; histochemistry; renal
tubules, toxicity.
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