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Immunohistochemical Study Effects of Spirulina Algae on the
Induced Mammary Tumor in Rats
Mashael Mohammed Bin-Meferij
College of Education, Scientific sections, Zoology Dept. Riyadh University For
Women, K.S.A
Abstract
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This work aimed at investigating the protective effects of Spirulina
platensis on the induced mammary tumor in rats by
dimethylbenz(a)anthracene (DMBA) and the proliferation of the tumor
cells by using immunohistochemical staining for proliferating cell
nuclear antigen (PCNA). At 50 days of age, group 1 remained untreated,
group 2 treated with 2% Spirulina platenesis in food, group 3 received
50 mg/kg DMBA i.p. groupe 4 received 50 mg/kg DMBA i.p and fed on 2%
spirulina. Rats were killed when the largest mammary tumor reached 1-2
cm in diameter or after 6 months of animal>s age. All the tumors
produced by DMBA were ductal carcinoma in 100% of group 3, but in group
4 two rats had mammary tumor. The groups 1 and 2 had no tumor and have
the same histological and immunostaining features, but in group 4, 13/15
rats had no tumor except formation of some cysts and hyperplasia in
epithelial cells. The conclusion of this work suggests that Spirulina
platnesis could be considered as a chemotherapeutic agent that causes
apoptosis to tumor cells by reducing the number of malignant cells and
resists cancer formation.
Key words: Breast cancer, Spirulina platensis, PCNA,
immunohistochemistry.
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