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Semen Quality of Holstein and Buffalo Bulls after
Filtration using Sephadex Column
Abdel-Khalek, A.E.*; Aboul-Ela, M.B.*; Soheir, A. Fawzy** and Dandooush E.**
*Dept. Animal Production., Faculty of Agriculture., Mansoura University, Egypt
**Animal. Production. Res. Inst., Agriculture. Research. Center, Egypt.
Abstract
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To evaluate the effect of sephadex column filtration technique on semen
quality of five Holstein bulls and five Egyptian buffalo bulls. Semen
was collected biweekly from each eight weeks. Immediately after
collection, semen was extended (37oC) and filtered using sephadex
column-filtration technique. Semen was evaluated for physical semen
characteristics including, percentages of sperm motility, live sperm and
sperm abnormality as well as sperm cell concentration pre-and
post-filtration. Results show that among all physical semen
characteristics, only ejaculate semen volume was significantly (P<0.001)
higher in Holstein than buffalo bulls, but motility, livability,
abnormality, sperm concentration and sperm with intact acrosome did not
differ between both species. As a result of filtration, sperm motility
and livability increased (P<0.05) by 16.4 and 11.8% in Holstein and by
16.9 and 10.1% in buffalo semen, respectively. Sperm abnormality and
concentration reduced (P<0.05) by 2.6 and 3.3% in Holstein and by 2.4
and 3.5% in buffalo semen, respectively. Improvements of live sperm and
the reduction in sperm concentration (proportional to the pre-filtration
value) were better (P<0.05) in Holstein than buffalo semen (15.5% and
%52.4 vs. 13.2 and –49.3%, respectively). Improvement of motility and
abnormality did not differ in Holstein (25.4 and %57.8) and buffalo
semen (26.6 and ,(%54.5respectively. The present results indicate that
using sephadex column filter technique has beneficial effects on
improving quality of spermatozoa in both species.
Key words: Holstein, buffaloes, semen, sephadex column filtration
technique.
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